This is a QCIF course and places are highly limited as Graduate School is paying the fee. If you are unable to attend, please de-register (or email graduateschool@uq.edu.au if you are unable to do so). An unexplained absence could result in all your future QCIF registrations becoming Waitlisted.

 

Genome annotation is crucial to defining the function of genomic sequences. This process typically involves a round of automated annotation followed by manual curation. Manual curation allows you to visualise your annotations so you can understand what your organism looks like, and then to manually refine these annotations along with any additional data you might have. This process is typically performed collaboratively as part of a team effort.

Apollo is a popular tool for facilitating real-time collaborative, manual curation and genome annotation editing. In this workshop we will learn how to use Apollo to refine genome annotations using example data from an E. coli strain. We’ll focus on the basics like getting data into Apollo, viewing evidence tracks, editing and adding structural and functional annotation, visualising the results and collaborating on genome annotations.

This workshop will make use of a training instance of the new Australian Apollo Service. This service enables Australian-based research groups and consortia to access Apollo and host genome assembly and supporting evidence files for free, and is operated by QCIF and the Australian BioCommons on computational infrastructure from the Pawsey Supercomputing Research Centre.

Recommended Participants

This workshop is for Australian researchers who have or will work on genome annotation and manual curation/editing as part of their projects. You should be familiar with the concepts of genome annotation and have, or soon have, genome annotation files that require manual annotation and curation.

Learning Objectives

By the end of this workshop you should be able to:

  • Upload data to Apollo
  • Visualise your genome and associated automated annotations 
  • Manually annotate genomes after automated annotations have been performed
  • Evaluate and visualise annotated genomic features
  • Use Apollo to collaborate on genome annotation

About Bioinformatics and genomics

If your research study involves bioinformatics and genomics, we've got you covered! The Graduate School works with different providers to provide a range of sessions with esteemed providers such as QCIF.

Useful links

  • Queensland Facility for Advanced Bioinformatics (QFAB): Bioinformatics, biostatistics and biodata expertise
  • Hacky Hour (supported by RCC, QCIF, IMB and wonderful volunteer helpers!): RCC runs consultations every Tuesday at Cafe Nano. Come and ask IT questions such as: getting started with Python and R, stats, machine learning, bio-image, software tools, research data management (RDM), how to access high performance computing, cloud data storage and tools for data cleaning and data visualisation. Bring your project's IT problems or come along as a helper! Very occasionally Hacky Hour may not run; follow us on Twitter (@HackyHourStLuc​) to get the latest announcements. Aside from the usual IT research questions, 
    • First Tuesday of the monthBioinformatics Hacky Hour, ask bioinformatics analysis, software and pipeline questions
    • Last Tuesday of the monthBioLab Hacky Hour, ask about experimental design and technical sample processing questions to get started in the lab