RNA Seq analysis using Galaxy (QCIF) [webinar]

(Workshop formerly known as RNA-Seq using Galaxy.)

This is a QCIF course and places are highly limited as Graduate School is paying the fee. If you are unable to attend, please de-register (or email graduateschool@uq.edu.au if you are unable to do so). An unexplained absence could result in all your future QCIF registrations becoming Waitlisted.

This interactive hands-on workshop introduces the concepts of RNA-Seq analysis, from data preparation through to statistical testing for differential gene expression, along with more advanced topics such as identification of novel transcription features and pathway and functional enrichment analysis. The workshop will focus on the use of Galaxy, a platform that provides a simple and user-friendly interface to bioinformatics tools, and will also feature an introduction to other freely-available RNA-Seq analysis resources. No prior experience required.

Learning Objectives

• Perform QC evaluation and filtering on next-generation sequencing data
• Use the Galaxy interface to identify differentially expressed genes from a RNA-Seq experiment
• Identify over-represented gene functions and pathways from a list of differentially expressed genes

Syllabus

• The tools and workflows for RNA-seq analysis
• Analysis of RNA-seq data for organisms with reference genomes
• Graphical summaries of gene expression data