This is a QFAB course and places are highly limited as Graduate School is paying the fee. If you are unable to attend, please de-register (or email graduateschool@uq.edu.au if you are unable to do so). An unexplained absence could result in all your future QFAB registrations becoming Waitlisted.

Syllabus

●Introduction to long read next-generation sequencing data

●Differences in genome assembly from short and long-read next-generation sequencing data

●Using SMRTLink to manage sample data

●Using SMRTLink to perform quality control filtering and genome assembly

●Visualisation of assembled genome in SMRTLink

●Export of genome for downstream analysis

●Assessment of genome completeness by use of comparison tools

Learning Objectives

●Perform QC evaluation and filtering on long read next-generation sequence data

●Use SMRTLink to assemble genome

●Visualise assembly using SMRTLink

●Assess genome quality using BUSCO and Augustus

●Export of genome data files for downstream analysis

 

About Bioinformatics and genomics

If your research study involves bioinformatics and genomics, we've got you covered! The Graduate School works with different providers to provide a range of sessions with esteemed providers such as QCIF.

Useful links

  • Queensland Facility for Advanced Bioinformatics (QFAB): Bioinformatics, biostatistics and biodata expertise
  • Hacky Hour (supported by RCC, QCIF, IMB and wonderful volunteer helpers!): RCC runs consultations every Tuesday at Cafe Nano. Come and ask IT questions such as: getting started with Python and R, stats, machine learning, bio-image, software tools, research data management (RDM), how to access high performance computing, cloud data storage and tools for data cleaning and data visualisation. Bring your project's IT problems or come along as a helper! Very occasionally Hacky Hour may not run; follow us on Twitter (@HackyHourStLuc​) to get the latest announcements. Aside from the usual IT research questions, 
    • First Tuesday of the monthBioinformatics Hacky Hour, ask bioinformatics analysis, software and pipeline questions
    • Last Tuesday of the monthBioLab Hacky Hour, ask about experimental design and technical sample processing questions to get started in the lab